Cynomolgus BST2 Protein (BST-CM202) Summary
Product Description |
Recombinant Cynomolgus BST2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile49-Ser163. |
Product Background |
Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus BST2 Protein (BST-CM202) Specifications/Details
Protein |
BST2 |
Synonyms |
BST-2; HM1.24 antigen; CD317; BST2; NPC-A-7; PDCA-1; TETHERIN |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ile49-Ser163 |
Molecular Weight |
The protein has a predicted MW of 13.78 kDa. Due to glycosylation, the protein migrates to 15-30 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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