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Cynomolgus IL-18RAP/IL-18 R beta Protein (IL8-CM1AP) Summary
Product Description |
Recombinant Cynomolgus IL-18RAP/IL-18 R beta Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Arg356. |
Product Background |
IL-18RAP, also known as IL-18Rβ or IL-1R7, is component of IL-18 receptor complex and its interactions with IL-18R1 mediate signal transduction initiated by IL-18 although it does not directly bind IL-18 in vitro. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus IL-18RAP/IL-18 R beta Protein (IL8-CM1AP) Specifications/Details
Protein |
IL-18RAP/IL-18 R beta |
Synonyms |
IL-18 R beta; ACPL; CD218b; CDw218b; IL-18R-beta; IL-18Rbeta; IL-18RAcP; IL-1R-7; IL-1R7; IL-1RAcPL; IL18RB; IL18RAP; IL-18R-beta; IL-18R-β; IL-18Rβ |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Phe20-Arg356 |
Molecular Weight |
The protein has a predicted MW of 39.47 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-18RAP, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Immobilized Cynomolgus IL-18RAP, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. |
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|
Cynomolgus IL-18RAP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Cynomolgus IL-18RAP is greater than 95% as determined by SEC-HPLC. |
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