Name: FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (MHC-HM43RTF)
Brand: KactusBio
SKU: MHC-HM43RTF-100UG
Price: 1150.0 USD
Availability: InStock

FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (MHC-HM43RTF)

Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.

Express System: HEK293
Product Tag: C-His
Activity: Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)

Catalog #MHC-HM43RTF

Size

100UG

500UG

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Product Description	Recombinant FITC-equivalent Human Peptide Ready HLA-A02:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A02:01) and Ile21-Met119 (B2M).
Product Background	Peptide Ready HLA-A02:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	Peptide Ready HLA-A*02:01&B2M Tetramer
Synonyms	MHC; HLA-A*02:01; Peptide Ready
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)
Molecular Weight	The protein has a predicted MW of 301.6 kDa.
Activity	Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Fluorophore	FITC
Expression System	HEK293
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.

Additional Information

Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure