FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (MHC-HM43RTF)

  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)

Catalog: MHC-HM43RTF

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Product Description Recombinant FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).

Product Background

Peptide Ready HLA-A*02:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein

Target

Peptide Ready HLA-A*02:01&B2M Tetramer
Accession
MHC Form Tetramer
Allele HLA-A*02:01

Species

Human

Exact Sequence

Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)
Molecular Weight The protein has a predicted MW of 301.6 kDa.

Activity

Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Fluorophore

FITC

Express System

HEK293
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.

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