FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (MHC-HM43RTF)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)
Catalog #MHC-HM43RTF
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Product Description
 Recombinant FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).
Product Background
 Peptide Ready HLA-A*02:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category
 Recombinant Protein
Target
 Peptide Ready HLA-A*02:01&B2M Tetramer
Synonyms
 MHC; HLA-A*02:01; Peptide Ready
MHC Form
 Tetramer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)
Molecular Weight
 The protein has a predicted MW of 301.6 kDa.
Activity
 Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Fluorophore
 FITC
Express System
 HEK293
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized FITC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.

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