Product Description |
Recombinant FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*03:01) and Ile21-Met119 (B2M). |
Product Background |
Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein |
Target |
Peptide Ready HLA-A*03:01&B2M |
MHC Form |
Tetramer |
Allele |
HLA-A*03:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 301.2 kDa. |
Activity |
Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Fluorophore |
FITC |
Express System |
HEK293 |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA. |
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