FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein (MHC-HM44RTF)
FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein (MHC-HM44RTF)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)
Catalog #MHC-HM44RTF
Regular price $1,150.00
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Product Description
Recombinant FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*03:01) and Ile21-Met119 (B2M).
Product Background
Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category
Recombinant Protein
Target
Peptide Ready HLA-A*03:01&B2M
MHC Form
Tetramer
Allele
HLA-A*03:01
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)
Molecular Weight
The protein has a predicted MW of 301.2 kDa.
Activity
Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Fluorophore
FITC
Express System
HEK293
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55ug/ml determined by ELISA.

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