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Human Annexin V/ANXA5 Protein (AN5-HE005) Summary
Product Description |
Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320. |
Product Background |
Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Annexin V/ANXA5 Protein (AN5-HE005) Specifications/Details
Protein |
Annexin V/ANXA5 |
Synonyms |
Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; PP4; PAP-I; VAC-alpha; ANX5; ENX2; ANXA5; HEL-S-7; RPRGL3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala2-Asp320 |
Molecular Weight |
The protein has a predicted MW of 35.9 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 240mM NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC. |
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