Human Annexin V/ANXA5 Protein (AN5-HE005)
Human Annexin V/ANXA5 Protein (AN5-HE005)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala2-Asp320
Catalog #AN5-HE005
Regular price $165.00
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Product Description
Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320.
Product Background
Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.
Product Category
Recombinant Protein
Target
Annexin V/ANXA5
Synonyms
Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; PP4; PAP-I; VAC-alpha; ANX5; ENX2; ANXA5; HEL-S-7; RPRGL3
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala2-Asp320
Molecular Weight
The protein has a predicted MW of 35.9 kDa same as Bis-Tris PAGE result.
Product Tag
No Tag
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM Tris, 240mM NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC.

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