Human Annexin V/ANXA5 Protein (AN5-HE005)
Human Annexin V/ANXA5 Protein (AN5-HE005)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala2-Asp320
Catalog #AN5-HE005
Regular price $165.00
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Product Description

Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320.

Product Background

Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

Annexin V/ANXA5

Synonyms

Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; PP4; PAP-I; VAC-alpha; ANX5; ENX2; ANXA5; HEL-S-7; RPRGL3

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala2-Asp320

Molecular Weight

The protein has a predicted MW of 35.9 kDa same as Bis-Tris PAGE result.

Product Tag

No Tag

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM Tris, 240mM NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC. The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC.

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