Human B7-H3 (4Ig) /B7-H3b Protein (BH7-HM43B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H3 (4Ig) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly27-Thr461

Catalog: BH7-HM43B

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Product Description Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly27-Thr461.
Product Category Recombinant Protein
Target B7-H3 (4Ig)/B7-H3b
Synonyms B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Gene/Protein ID Q5ZPR3-1
Species Human
Exact Sequence Gly27-Thr461
Molecular Weight The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-H3 (4Ig) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H3 (4Ig) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA.
The purity of Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.
Human B7-H3 (4Ig) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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