Human CD200 R1/CRTR2 Protein (CD2-HM2R1)
Human CD200 R1/CRTR2 Protein (CD2-HM2R1)
Human CD200 R1/CRTR2 Protein (CD2-HM2R1)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Leu266
  • Other Available Proteins:
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Catalog #CD2-HM2R1
Regular price $297.00
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Size
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Human CD200 R1/CRTR2 Protein (CD2-HM2R1) Summary

Product Description

Recombinant Human CD200 R1/CRTR2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala27-Leu266.

Product Background

CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CD200 R1/CRTR2 Protein (CD2-HM2R1) Specifications/Details

Protein
CD200 R1/CRTR2
Synonyms
CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala27-Leu266
Molecular Weight
The protein has a predicted MW of 53.7 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA.
Product Tag
C-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA. Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA.
Human CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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