- Express System: HEK293
- Product Tag: C-hFc
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ala27-Leu266
Product Description |
Recombinant Human CD200 R1/CRTR2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Leu266. |
Product Background |
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
CD200 R1/CRTR2 |
Synonyms |
CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Leu266 |
Molecular Weight |
The protein has a predicted MW of 53.7 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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