- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA (QC Test).
- Amino Acid Range: Asp35-Ser353
-
Other Available Proteins:
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Human CD55 Protein (CD5-HM105) Summary
Product Description |
Recombinant Human CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp35-Ser353. |
Product Background |
Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD55 Protein (CD5-HM105) Specifications/Details
Protein |
CD55 |
Synonyms |
DAF-GPI; CD55; Cd55a; Daf; Daf1; DAF; CR; CROM; TC |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp35-Ser353 |
Molecular Weight |
The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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