Product Description |
Recombinant Human CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp35-Ser353. |
Product Background |
Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. |
Product Category |
Recombinant Protein |
Target |
CD55 |
Synonyms |
DAF-GPI; CD55; Cd55a; Daf; Daf1; DAF; CR; CROM; TC |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp35-Ser353 |
Molecular Weight |
The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. |
|
|
Human CD55 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD55 is greater than 95% as determined by SEC-HPLC. |
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