Human CD55 Protein (CD5-HM105)
Human CD55 Protein (CD5-HM105)
Human CD55 Protein (CD5-HM105)
Human CD55 Protein (CD5-HM105)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp35-Ser353
Catalog #CD5-HM105
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Product Description
 Recombinant Human CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp35-Ser353.
Product Background
 Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.
Product Category
 Recombinant Protein
Target
 CD55
Synonyms
 DAF-GPI; CD55; Cd55a; Daf; Daf1; DAF; CR; CROM; TC
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp35-Ser353
Molecular Weight
 The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
Human CD55 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD55 is greater than 95% as determined by SEC-HPLC.
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