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Human CDCP1 ATF&CTF Protein (CDC-HM104) Summary
Product Description |
Recombinant Human CDCP1 ATF&CTF Protein is expressed from HEK293 with HA tag at the N-terminus of ATF and His tag at the C-terminus of CTF.It contains Phe30-Arg368 (ATF) and Lys369-Asp665 (CTF). |
Product Background |
Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CDCP1 ATF&CTF Protein (CDC-HM104) Specifications/Details
Protein |
CDCP1 |
Synonyms |
CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe30-Arg368(ATF) & Lys369-Asp665(CTF) |
Molecular Weight |
The protein has a predicted MW of 39.16 kDa (ATF) and 35.30 kDa (CTF). Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CDCP1 ATF&CTF, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA. |
Product Tag |
N-HA, C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CDCP1 ATF&CTF, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA. |
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Human CDCP1 ATF&CTF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CDCP1 ATF&CTF is greater than 90% and the molecular weight of this protein is around 80-101 kDa as determined by SEC-MALS. |
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The purity of Human CDCP1 ATF&CTF is greater than 85% as determined by SEC-HPLC. |
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