• Express System: HEK293
  • Product Tag: N-HA, C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Human CDCP1 ATF&CTF, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA.
  • Amino Acid Range: Phe30-Arg368(ATF) & Lys369-Asp665(CTF)
Catalog #CDC-HM104
Price
Regular price $45000
Regular price Sale price $45000
/
Size
Avantor VWR Fisher Scientific

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Human CDCP1 ATF&CTF Protein (CDC-HM104) Summary

Product Description

 Recombinant Human CDCP1 ATF&CTF Protein is expressed from HEK293 with HA tag at the N-terminus of ATF and His tag at the C-terminus of CTF.It contains Phe30-Arg368 (ATF) and Lys369-Asp665 (CTF).

Product Background

 Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Human CDCP1 ATF&CTF Protein (CDC-HM104) Specifications/Details

Protein
 CDCP1
Synonyms
 CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe30-Arg368(ATF) & Lys369-Asp665(CTF)
Molecular Weight
 The protein has a predicted MW of 39.16 kDa (ATF) and 35.30 kDa (CTF). Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CDCP1 ATF&CTF, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA.
Product Tag
 N-HA, C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CDCP1 ATF&CTF, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA. Immobilized Human CDCP1 ATF&CTF, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA.
Human CDCP1 ATF&CTF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CDCP1 ATF&CTF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CDCP1 ATF&CTF is greater than 90% and the molecular weight of this protein is around 80-101 kDa as determined by SEC-MALS. The purity of Human CDCP1 ATF&CTF is greater than 90% and the molecular weight of this protein is around 80-101 kDa as determined by SEC-MALS.
The purity of Human CDCP1 ATF&CTF is greater than 85% as determined by SEC-HPLC. The purity of Human CDCP1 ATF&CTF is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...