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Human FAP Protein (active dimer) (FAP-HM101) Summary
Product Description |
Recombinant Human FAP Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu26-Asp760. |
Product Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets |
Human FAP Protein (active dimer) (FAP-HM101) Specifications/Details
Protein |
FAP |
Synonyms |
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Asp760 |
Molecular Weight |
The protein has a predicted MW of 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FAP, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >7000-pmol/min/µg (QC Test). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human FAP, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). |
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Human FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human FAP is greater than 95% and the molecular weight of this protein is around 175-200 kDa as determined by SEC-MALS. |
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The purity of Human FAP is greater than 95% as determined by SEC-HPLC. |
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