Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
Human FAP Protein (FAP-HM101)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FAP, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu26-Asp760
Catalog #FAP-HM101
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Product Description
 Recombinant Human FAP Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu26-Asp760.
Product Background
 Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Product Category
 Recombinant Protein
Target
 FAP
Synonyms
 FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu26-Asp760
Molecular Weight
 The protein has a predicted MW of 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human FAP, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris,0.25M NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FAP, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
Human FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human FAP is greater than 95% as determined by SEC-HPLC.
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