Human FAP Protein (FAP-HM101)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu26-Asp760

Catalog: FAP-HM101

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Product Description Recombinant Human FAP Protein is expressed from Expi293 with His tag at the N-terminal.It contains Leu26-Asp760.
Product Category Recombinant Protein
Target FAP
Synonyms FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Gene/Protein ID Q12884-1
Species Human
Exact Sequence Leu26-Asp760
Molecular Weight The protein has a predicted MW of 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 250mM NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA.
The purity of Human FAP is greater than 95% as determined by SEC-HPLC.
Human FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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