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Human GPC3/Glypican 3 Protein, Ultra Low Endotoxin (GPC-HM131-UL) Summary
Product Description |
Recombinant Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559. |
Product Background |
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed. |
Product Category |
Recombinant Protein / Antibody discovery, Cell therapy / ADC targets |
Human GPC3/Glypican 3 Protein, Ultra Low Endotoxin (GPC-HM131-UL) Specifications/Details
Protein |
GPC3/Glypican 3 |
Synonyms |
GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln25-His559 |
Molecular Weight |
The protein has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 30-31 kDa, 38-42 kDa and 65-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). |
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Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-GPC3 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human GPC3, His Tag with an affinity constant of 1.72 nM as determined in BLI assay . |
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The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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