Name: Human PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-HM110-UL)
Brand: KactusBio
SKU: PSM-HM110-UL-100UG
Price: 417.0 USD
Availability: InStock

Human PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-HM110-UL)

Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.

Human PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

The purity of Human PSMA is greater than 95% and the molecular weight of this protein is around 170-220 kDa as determined by SEC-MALS.

Loaded Anti-PSMA Ab., hFc Tag on ProA-Biosensor can bind Human PSMA, His Tag with an affinity constant of 1.74 nM as determined in BLI assay (Gator® Prime).

The purity of Human PSMA is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His
Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >400 pmol/min/µg.
Amino Acid Range: Lys44-Ala750

Catalog #PSM-HM110-UL

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-HM110-UL) Summary

Product Description	Recombinant Human PSMA/FOLH1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys44-Ala750.
Product Background	Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Human PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-HM110-UL) Specifications/Details

Protein	PSMA/FOLH1
Synonyms	GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
Accession	Q04609-1
Species	Human
Biotinylated	no
Amino Acid Range	Lys44-Ala750
Molecular Weight	The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >400 pmol/min/µg.
Product Tag	N-His
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0).
Reconstitution	Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
	Human PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Human PSMA is greater than 95% and the molecular weight of this protein is around 170-220 kDa as determined by SEC-MALS.
	Loaded Anti-PSMA Ab., hFc Tag on ProA-Biosensor can bind Human PSMA, His Tag with an affinity constant of 1.74 nM as determined in BLI assay (Gator® Prime).
	The purity of Human PSMA is greater than 95% as determined by SEC-HPLC.