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Human DLL3 Protein, Ultra Low Endotoxin (DLL-HM103-UL) Summary
Product Description |
Recombinant Human DLL3 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Ala27-Arg490. |
Product Background |
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis and may be required to divert neurons along a specific differentiation pathway. It plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human DLL3 Protein, Ultra Low Endotoxin (DLL-HM103-UL) Specifications/Details
Protein |
DLL3 |
Synonyms |
Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Arg490 |
Molecular Weight |
The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DLL3, His Tag at 0.1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Anti-DLL3 Antibody captured on CM5 Chip via Protein A can bind Human DLL3, His Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human DLL3, His Tag at 0.1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA (QC Test). |
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Human DLL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3, His Tag with an affinity constant of 0.35 nM as determined in BLI assay . |
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The purity of Human DLL3 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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