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Human CDCP1 Protein, Ultra Low Endotoxin (CDC-HM101-UL) Summary
Product Description |
Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Leu666. |
Product Background |
Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CDCP1 Protein, Ultra Low Endotoxin (CDC-HM101-UL) Specifications/Details
Protein |
CDCP1 |
Synonyms |
CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe30-Leu666 |
Molecular Weight |
The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human CDCP1, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200). |
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Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). |
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Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CDCP1 is greater than 95% and the molecular weight of this protein is around 80-105 kDa as determined by SEC-MALS. |
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Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human CDCP1, His Tag with an affinity constant of 17.00 nM as determined in BLI assay . |
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The purity of Human CDCP1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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