Name: Human HMGB1 Protein (HMG-HM1B1)
Brand: KactusBio
SKU: HMG-HM1B1-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.19 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met1-Glu215
Other Available Proteins:

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Catalog #HMG-HM1B1

Size

100UG

500UG

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Human HMGB1 Protein (HMG-HM1B1) Summary

Product Description	Recombinant Human HMGB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215.
Product Background	High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human HMGB1 Protein (HMG-HM1B1) Specifications/Details

Protein	HMGB1
Synonyms	HMGB1; HMG1; HMG3; SBP-1; HMG-1
Accession	P09429-1
Species	Human
Biotinylated	no
Amino Acid Range	Met1-Glu215
Molecular Weight	The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.19 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human AGER, His Tag immobilized on CM5 Chip can bind Human HMGB1, His Tag with an affinity constant of 0.19 uM as determined in SPR assay (Biacore T200).
	Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HMGB1 is greater than 95% as determined by SEC-HPLC.

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