Human IFN alpha/beta R2 Protein (IFN-HM10R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ile27-Lys243
  • Other Available Proteins:
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Catalog #IFN-HM10R
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Human IFN alpha/beta R2 Protein (IFN-HM10R) Summary

Product Description

 Recombinant Human IFN alpha/beta R2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile27-Lys243.

Product Background

 Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IFN alpha/beta R2 Protein (IFN-HM10R) Specifications/Details

Protein
 IFN alpha/beta R2
Synonyms
 IFN-R-2; IFNAR2; IFNARB; IFNABR; IFN-alpha; IFN-alpha/beta R2; IFNbR2; IFN-R; β receptor 2; β receptor10;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile27-Lys243
Molecular Weight
 The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200). Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).
Human IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator). Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator).
The purity of Human IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC. The purity of Human IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.
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