Product Description |
Recombinant Human LRP-5-Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Glu644-Gln1263. |
Product Background |
A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis. |
Product Category |
Recombinant Protein |
Target |
LRP-5 |
Synonyms |
LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu644-Gln1263 |
Molecular Weight |
The protein has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Wnt Surrogate-Fc Fusion, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.73ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-mFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Wnt Surrogate-Fc Fusion, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.73ug/ml determined by ELISA (QC Test). |
|
|
Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.63ug/ml determined by ELISA. |
|
|
Human LRP-5-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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