Human LRP-6-Protein (LRP-HM306)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 90% as determined by Tris-Bis PAGE
  • Activity: Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Pro630

Catalog: LRP-HM306

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Product Description Recombinant Human LRP-6-Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Pro630.

Product Background

Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.

Product Category

Recombinant Protein

Target

LRP-6
Synonyms LRP-6; DCAD2; FLJ90062; FLJ90421
Accession

Species

Human

Amino Acid Range

Ala20-Pro630
Molecular Weight The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 90% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
Human LRP-6-on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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