Name: Human PD-1/PDCD1 Protein (PD1-HM201)
Brand: KactusBio
SKU: PD1-HM201-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-L1, mFc Tag (Cat. PDL-HM310) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu25-Gln167
Other Available Proteins:

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Catalog #PD1-HM201

Size

100UG

500UG

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Human PD-1/PDCD1 Protein (PD1-HM201) Summary

Product Description	Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.
Product Background	Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human PD-1/PDCD1 Protein (PD1-HM201) Specifications/Details

Protein	PD-1/PDCD1
Synonyms	PDCD1; PD1; CD279; SLEB2; PD-1
Accession	Q15116-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Gln167
Molecular Weight	The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PD-L1, mFc Tag (Cat. PDL-HM310) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag (Cat. PDL-HM110) with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
	Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag (Cat. PDL-HM402B) with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
	Immobilized Human PD-L1, mFc Tag (Cat. PDL-HM310) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test).
	Immobilized Human PD-1, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA.
	Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.
	Serial dilutions of Anti-PD-1 Antibody were added into Human PD-1, hFc Tag: Biotinylated Human PD-L1, His Avi Tag (Cat. PDL-HM410B) binding reaction. The half maximal inhibitiory concentration (IC50) is 0.22ug/ml.
	Loaded Human PD-1, hFc Tag on ProA-Biosensor can bind Human PD-L2, His-Avi Tag (Cat. PDL-HM402) with an affinity constant of 0.32 µM as determined in BLI assay .
	The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.

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