Human PD-1/PDCD1 Protein (PD1-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2ng/ml determined by ELISA. The affinity constant of 0.344 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu25-Gln167

Catalog: PD1-HM201

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Product Description Recombinant Human PD-1/PDCD1 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Leu25-Gln167 .
Product Category Recombinant Protein
Target PD-1/PDCD1
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID Q15116-1
Species Human
Exact Sequence Leu25-Gln167
Molecular Weight The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2ng/ml determined by ELISA. The affinity constant of 0.344 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 μM as determined in SPR assay (Biacore T200).
Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2ng/ml determined by ELISA.
Immobilized Human PD-1, hFc Tag at 1μg/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1μg/ml determined by ELISA.
Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2/30.0/30.7/33.4ng/ml determined by ELISA.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.

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