Human PD-1/PDCD1 Protein (PD1-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-1, hFc Tag at 1ug/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1ug/ml determined by ELISA. The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu25-Gln167

Catalog: PD1-HM201

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Product Description	Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Gln167.
Product Background	Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Product Category	Recombinant Protein

Target	PD-1/PDCD1
Synonyms	PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID	Q15116-1
Species	Human
Exact Sequence	Leu25-Gln167
Molecular Weight	The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PD-1, hFc Tag at 1ug/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1ug/ml determined by ELISA. The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
	Immobilized Human PD-1, hFc Tag at 1ug/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1ug/ml determined by ELISA.
	Immobilized Human PD-1, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test).
	The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
	Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.