Human PD-1/PDCD1 Protein (PD1-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu25-Gln167

Catalog: PD1-HM201

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Product Description Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Gln167.

Product Background

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Product Category

Recombinant Protein

Target

PD-1/PDCD1
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
Accession

Species

Human

Amino Acid Range

Leu25-Gln167
Molecular Weight The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test).
Immobilized Human PD-1, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.

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