Product Description |
Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Gln167. |
Product Background |
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. |
Product Category |
Recombinant Protein |
Target |
PD-1/PDCD1 |
Synonyms |
PDCD1; PD1; CD279; SLEB2; PD-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu25-Gln167 |
Molecular Weight |
The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). |
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Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). |
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Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test). |
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Immobilized Human PD-1, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. |
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Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%. |
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The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC. |
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