Name: Human PD-L1/B7-H1 Protein (PDL-HM110)
Brand: KactusBio
SKU: PDL-HM110-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA. The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
Amino Acid Range: Phe19-Arg238
Other Available Proteins:

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Catalog #PDL-HM110

Size

100UG

500UG

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Human PD-L1/B7-H1 Protein (PDL-HM110) Summary

Product Description	Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.
Product Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins \| ADC targets

Human PD-L1/B7-H1 Protein (PDL-HM110) Specifications/Details

Protein	PD-L1/B7-H1
Synonyms	CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession	Q9NZQ7-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe19-Arg238
Molecular Weight	The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA. The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
	Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA.
	Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-PD-L1 Antibody were added into Human PD-L1, His Tag : Biotinylated Human PD-1, hFc Avi Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 11.7ng/ml.
	Loaded Biotinylated Human PD-1, His Tag on Streptavidin-Biosensor can bind Human PD-L1, His Tag with an affinity constant of 0.20 µM as determined in BLI assay .
	The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

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