Name: Human PD-L1/B7-H1 Protein (PDL-HM110)
Brand: KactusBio
SKU: PDL-HM110-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA. The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe19-Arg238

Catalog #PDL-HM110

Size

100UG

500UG

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Product Description	Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.
Product Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	PD-L1/B7-H1
Synonyms	CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession	Q9NZQ7-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe19-Arg238
Molecular Weight	The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA. The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
	Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA.
	Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.