Name: Human RNF43 Protein (RNF-HM143)
Brand: KactusBio
SKU: RNF-HM143-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.255 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly24-Tyr197

Catalog #RNF-HM143

Size

100UG

500UG

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Human RNF43 Protein (RNF-HM143) Summary

Product Description	Recombinant Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly24-Tyr197.
Product Background	RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Human RNF43 Protein (RNF-HM143) Specifications/Details

Protein	RNF43
Synonyms	E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43
Accession	Q68DV7-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly24-Tyr197
Molecular Weight	The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.255 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 uM as determined in SPR assay (Biacore T200).
	Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.

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