Product Description |
Recombinant Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly24-Tyr197. |
Product Background |
RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation. |
Product Category |
Recombinant Protein |
Protein |
RNF43 |
Synonyms |
E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly24-Tyr197 |
Molecular Weight |
The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.255 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 uM as determined in SPR assay (Biacore T200). |
|
|
Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC. |
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