Human RNF43 Protein (RNF-HM143)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.255 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly24-Tyr197

Catalog: RNF-HM143

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Product Description	Recombinant Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly24-Tyr197.
Product Background	RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Product Category	Recombinant Protein

Target	RNF43
Gene/Protein ID	Q68DV7-1
Species	Human
Exact Sequence	Gly24-Tyr197
Molecular Weight	The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.255 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 uM as determined in SPR assay (Biacore T200).
	The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.
	Human RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.