Name: Human TEM1/cd248 Protein (TEM-HM148)
Brand: KactusBio
SKU: TEM-HM148-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Amino Acid Range: Gln18-Leu687
Other Available Proteins:

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Catalog #TEM-HM148

Size

100UG

500UG

Order by 4pm ET for delivery on:

Human TEM1/cd248 Protein (TEM-HM148) Summary

Product Description	Recombinant Human TEM1/cd248 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Leu687.
Product Background	Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human TEM1/cd248 Protein (TEM-HM148) Specifications/Details

Protein	TEM1/cd248
Synonyms	Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479
Accession	Q9HCU0-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln18-Leu687
Molecular Weight	The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 125-155 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
	Human TEM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Human TEM1 is greater than 90% as determined by SEC-HPLC.

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