Human TEM1/cd248 Protein (TEM-HM148)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln18-Leu687

Catalog: TEM-HM148

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Product Description Recombinant Human TEM1/cd248 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Leu687.

Product Background

Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.

Product Category

Recombinant Protein

Target

TEM1/cd248
Synonyms Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479
Accession

Species

Human

Amino Acid Range

Gln18-Leu687
Molecular Weight The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
The purity of Human TEM1 is greater than 95% as determined by SEC-HPLC.
Human TEM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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