Name: Human TEM1/cd248 Protein (TEM-HM148)
Brand: KactusBio
SKU: TEM-HM148-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln18-Leu687

Catalog #TEM-HM148

Size

100UG

500UG

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Product Description	Recombinant Human TEM1/cd248 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Leu687.
Product Background	Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.
Product Category	Recombinant Protein / Antibody discovery

Protein	TEM1/cd248
Synonyms	Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479
Accession	Q9HCU0-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln18-Leu687
Molecular Weight	The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
	Human TEM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TEM1 is greater than 95% as determined by SEC-HPLC.