Product Description |
Recombinant Human TRAIL Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly118-Gly281. |
Product Background |
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents. |
Product Category |
Recombinant Protein |
Target |
Trail Trimer |
Synonyms |
Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly118-Gly281 |
Molecular Weight |
The protein has a predicted MW of 60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TRAIL Trimer, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human TRAIL Trimer, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. |
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Human TRAIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TRAIL Trimer is greater than 90% as determined by SEC-HPLC. |
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