Human TRAIL Trimer Protein (TRL-HM101)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Human TRAIL Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly118-Gly281

Catalog: TRL-HM101

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Product Description	Recombinant Human TRAIL Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly118-Gly281.
Product Background	Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.
Product Category	Recombinant Protein

Target	Trail Trimer
Synonyms	Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2
Gene/Protein ID	P50591-1
Species	Human
Exact Sequence	Gly118-Gly281
Molecular Weight	The protein has a predicted MW of 60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TRAIL Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human TRAIL Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
	The purity of Human TRAIL Trimer is greater than 90% as determined by SEC-HPLC.
	Human TRAIL Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.