- Express System: HEK293
- Product Tag: N-His-Flag
- Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
- Activity: Immobilized Human TRAIL Trimer, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Gly118-Gly281
Product Description |
Recombinant Human TRAIL Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly118-Gly281. |
Product Background |
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
TRAIL Trimer |
Synonyms |
Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly118-Gly281 |
Molecular Weight |
The protein has a predicted MW of 60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TRAIL Trimer, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed