HPLC chromatogram showing a single sharp peak at 9.362 minutes, indicating high purity of the recombinant protein sample.
Mouse ASPH Protein (ASP-ME101)
HPLC chromatogram showing a single sharp peak at 9.362 minutes, indicating high purity of the recombinant protein sample.
Mouse ASPH Protein (ASP-ME101)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ile324-Ile741
  • Other Available Proteins:
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Catalog #ASP-ME101
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Size
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Mouse ASPH Protein (ASP-ME101) Summary

Product Description

 Recombinant Mouse ASPH Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ile324-Ile741.

Product Background

 Aspartate β-hydroxylase (ASPH) is silent in normal adult tissues only to re-emerge during oncogenesis where its function is required for generation and maintenance of malignant phenotypes. Exosomes enable prooncogenic secretome delivering and trafficking for long-distance cell-to-cell communication.Expression profiling of Notch signaling components positively correlates with ASPH expression in breast cancer patients, confirming that ASPH-Notch axis acts functionally in breast tumorigenesis.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse ASPH Protein (ASP-ME101) Specifications/Details

Protein
 ASPH
Synonyms
 Aspartate beta-hydroxylase; ASP beta-hydroxylase; Asph; Bah;Aspartate β-hydroxylase; ASP β-hydroxylase
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ile324-Ile741
Molecular Weight
 The protein has a predicted MW of 49.4 kDa same as Bis-Tris PAGE result.
Product Tag
 N-His

Fluorophore

 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse ASPH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse ASPH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse ASPH is greater than 95% as determined by SEC-HPLC. The purity of Mouse ASPH is greater than 95% as determined by SEC-HPLC.
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support@kactusbio.us

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