Name: Mouse B7-H2/ICOSLG Protein (BH7-MM172)
Brand: KactusBio
SKU: BH7-MM172-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Glu47-Lys279
Other Available Proteins:

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Catalog #BH7-MM172

Size

100UG

500UG

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Mouse B7-H2/ICOSLG Protein (BH7-MM172) Summary

Product Description	Recombinant Mouse B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu47-Lys279.
Product Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse B7-H2/ICOSLG Protein (BH7-MM172) Specifications/Details

Protein	B7-H2/ICOSLG
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession	Q9JHJ8-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Glu47-Lys279
Molecular Weight	The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
	Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA.
	Mouse B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse B7-H2 is greater than 95% as determined by SEC-HPLC.

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