• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu47-Lys279
Catalog #BH7-MM172
Price
Regular price $29700
Regular price Sale price $29700
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Size
Avantor VWR Fisher Scientific

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Mouse B7-H2/ICOSLG Protein (BH7-MM172) Summary

Product Description

 Recombinant Mouse B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu47-Lys279.

Product Background

 B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.

Mouse B7-H2/ICOSLG Protein (BH7-MM172) Specifications/Details

Protein
 B7-H2/ICOSLG
Synonyms
 CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50;B7H2B7-related protein 1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Glu47-Lys279
Molecular Weight
 The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200). Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA.
Mouse B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse B7-H2 is greater than 90% as determined by SEC-HPLC. The purity of Mouse B7-H2 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
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