Mouse B7-H2/ICOSLG Protein (BH7-MM172)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu47-Lys279

Catalog: BH7-MM172

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Product Description	Recombinant Mouse B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu47-Lys279.
Product Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Product Category	Recombinant Protein

Target	B7-H2/ICOSLG
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Gene/Protein ID	Q9JHJ8-1
Species	Mouse
Exact Sequence	Glu47-Lys279
Molecular Weight	The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
	The purity of Mouse B7-H2 is greater than 95% as determined by SEC-HPLC.
	Mouse B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.