Mouse B7-H2/ICOSLG Protein (BH7-MM172)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.329 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu47-Lys279

Catalog: BH7-MM172

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Product Description Recombinant Mouse B7-H2/ICOSLG Protein is expressed from Expi293 with His tag at the C-terminal.It contains Glu47-Lys279.
Product Category Recombinant Protein
Target B7-H2/ICOSLG
Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Gene/Protein ID Q9JHJ8-1
Species Mouse
Exact Sequence Glu47-Lys279
Molecular Weight The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.329 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 μM as determined in SPR assay (Biacore T200).
The purity of Mouse B7-H2 is greater than 95% as determined by SEC-HPLC.
Mouse B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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