Mouse CD200 R1/CRTR2 Protein (CD2-MM4R1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD200R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 96.7ng/ml determined by ELISA. The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Thr26-Pro238
  • Other Available Proteins:
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Catalog #CD2-MM4R1
Regular price $346.50
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Mouse CD200 R1/CRTR2 Protein (CD2-MM4R1) Summary

Product Description

Recombinant Mouse CD200 R1/CRTR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr26-Pro238.

Product Background

CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Mouse CD200 R1/CRTR2 Protein (CD2-MM4R1) Specifications/Details

Protein
CD200 R1/CRTR2
Synonyms
CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Thr26-Pro238
Molecular Weight
The protein has a predicted MW of 24.21 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD200R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 96.7ng/ml determined by ELISA. The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 uM as determined in SPR assay (Biacore T200). Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse CD200R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 96.7ng/ml determined by ELISA. Immobilized Mouse CD200R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 96.7ng/ml determined by ELISA.
Mouse CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD200 R1 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD200 R1 is greater than 95% as determined by SEC-HPLC.
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