Product Description |
Recombinant Mouse CD200 R1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr26-Pro238. |
Product Background |
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand. |
Product Category |
Recombinant Protein |
Target |
CD200 R1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Thr26-Pro238 |
Molecular Weight |
The protein has a predicted MW of 24.21 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse CD200R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.53ug/ml determined by ELISA (QC Test). The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 uM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse CD200R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.53ug/ml determined by ELISA (QC Test). |
|
|
Mouse CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse CD200 R1 is greater than 95% as determined by SEC-HPLC. |
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