Mouse CD200 R1 Protein (CD2-MM4R1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse CD200R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.53ug/ml determined by ELISA (QC Test). The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Thr26-Pro238

Catalog: CD2-MM4R1

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Product Description	Recombinant Mouse CD200 R1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr26-Pro238.
Product Background	CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Product Category	Recombinant Protein

Target	CD200 R1
Gene/Protein ID	Q9ES57
Species	Mouse
Exact Sequence	Thr26-Pro238
Molecular Weight	The protein has a predicted MW of 24.21 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse CD200R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.53ug/ml determined by ELISA (QC Test). The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
	Immobilized Mouse CD200R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.53ug/ml determined by ELISA (QC Test).
	The purity of Mouse CD200 R1 is greater than 95% as determined by SEC-HPLC.
	Mouse CD200 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.