Name: Mouse CD40/TNFRSF5 Protein (CD4-MM140)
Brand: KactusBio
SKU: CD4-MM140-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse CD40, His Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Val24-Arg193
Other Available Proteins:

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Catalog #CD4-MM140

Size

100UG

500UG

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Mouse CD40/TNFRSF5 Protein (CD4-MM140) Summary

Product Description	Recombinant Mouse CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Arg193.
Product Background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse CD40/TNFRSF5 Protein (CD4-MM140) Specifications/Details

Protein	CD40/TNFRSF5
Synonyms	CD40; CD40L receptor;- TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013;
Accession	P27512-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Val24-Arg193
Molecular Weight	The protein has a predicted MW of 19.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse CD40, His Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse CD40, His Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test).
	Mouse CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay .
	The purity of Mouse CD40 is greater than 95% as determined by SEC-HPLC.

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