Mouse CD40/TNFRSF5 Protein (CD4-MM140)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val24-Arg193

Catalog: CD4-MM140

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Product Description Recombinant Mouse CD40/TNFRSF5 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Val24-Arg193.
Product Category Recombinant Protein
Target CD40/TNFRSF5
Synonyms CD40; CD40L receptor;   TNFRSF5; B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
Gene/Protein ID P27512-1
Species Mouse
Exact Sequence Val24-Arg193
Molecular Weight The protein has a predicted MW of 19.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA.
The purity of Mouse CD40 is greater than 95% as determined by SEC-HPLC.
Mouse CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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