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Mouse IL-18 R1/CD218a Protein (IL8-MM1R1) Summary
Product Description |
Recombinant Mouse IL-18 R1/CD218a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys20-Gly326. |
Product Background |
Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse IL-18 R1/CD218a Protein (IL8-MM1R1) Specifications/Details
Protein |
IL-18 R1/CD218a |
Synonyms |
CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Lys20-Gly326 |
Molecular Weight |
The protein has a predicted MW of 36.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.30 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.30 uM as determined in SPR assay (Biacore T200). |
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Mouse IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IL-18 R1 is greater than 95% as determined by SEC-HPLC. |
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