Mouse IL-18 R1/CD218a Protein (IL8-MM1R1)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.30 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Lys20-Gly326

Catalog: IL8-MM1R1

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Product Description Recombinant Mouse IL-18 R1/CD218a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys20-Gly326.

Product Background

Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Product Category

Recombinant Protein

Target

IL-18 R1/CD218a
Synonyms CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra
Accession

Species

Mouse

Amino Acid Range

Lys20-Gly326
Molecular Weight The protein has a predicted MW of 36.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.30 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.30 uM as determined in SPR assay (Biacore T200).
The purity of Mouse IL-18 R1 is greater than 95% as determined by SEC-HPLC.
Mouse IL-18 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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