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Mouse IL-6 R alpha/CD126 Protein (ILR-MM16R) Summary
Product Description |
Recombinant Mouse IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu20-Pro364. |
Product Background |
The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse IL-6 R alpha/CD126 Protein (ILR-MM16R) Specifications/Details
Protein |
IL-6 R alpha/CD126 |
Synonyms |
IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu20-Pro364 |
Molecular Weight |
The protein has a predicted MW of 39.21 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.15 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse IL-6 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-6, His Tag (Cat. IL6-MM106) with an affinity constant of 0.15 uM as determined in SPR assay (Biacore T200). |
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Mouse IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IL-6 R alpha is greater than 95% as determined by SEC-HPLC. |
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