- Express System: HEK293
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test).
- Amino Acid Range: Lys33-Glu262
-
Other Available Proteins:
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Mouse M-CSF/CSF-1 Protein (CSF-MM001) Summary
Product Description |
Recombinant Mouse M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Lys33-Glu262. |
Product Background |
CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse M-CSF/CSF-1 Protein (CSF-MM001) Specifications/Details
Protein |
M-CSF/CSF-1 |
Synonyms |
CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Lys33-Glu262 |
Molecular Weight |
The protein has a predicted MW of 25.98 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test). |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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