- Express System: HEK293
- Product Tag: No Tag
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Lys33-Glu262
Product Description |
Recombinant Mouse M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Lys33-Glu262. |
Product Background |
CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
Product Category |
Recombinant Protein |
Target |
M-CSF/CSF-1 |
Synonyms |
CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Lys33-Glu262 |
Molecular Weight |
The protein has a predicted MW of 25.98 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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