Mouse M-CSF/CSF-1 Protein (CSF-MM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys33-Glu262
Catalog #CSF-MM001
Regular price $300.00
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Product Description
Recombinant Mouse M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Lys33-Glu262.
Product Background
CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Product Category
Recombinant Protein
Target
M-CSF/CSF-1
Synonyms
CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Lys33-Glu262
Molecular Weight
The protein has a predicted MW of 25.98 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA. See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 0.1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA.
Mouse M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse M-CSF is greater than 95% as determined by SEC-HPLC.

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