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Mouse Siglec-3/CD33 Protein (CD3-MM133) Summary
Product Description |
Recombinant Mouse Siglec-3/CD33 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp18-Glu240. |
Product Background |
CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. CD33 is the smallest siglec member. It preferentially binds to α2-6- and α2-3-sialylated glycans and strongly binds to sialylated ligands on leukemic cell lines. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Mouse Siglec-3/CD33 Protein (CD3-MM133) Specifications/Details
Protein |
Siglec-3/CD33 |
Synonyms |
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Asp18-Glu240 |
Molecular Weight |
The protein has a predicted MW of 25.72 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse Siglec-3 is greater than 95% as determined by SEC-HPLC. |
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