Bis-Tris PAGE gel image showing molecular weight markers in lane MK and purified Mouse TFPI-2 protein in lane R, with a prominent band observed between 40-50 kDa, indicating high purity.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Leu23-Ser230
  • Other Available Proteins:
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Catalog #TFP-MM102
Regular price $379.50
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Size
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Mouse TFPI-2 Protein (TFP-MM102) Summary

Product Description

Recombinant Mouse TFPI-2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu23-Ser230.

Product Background

Tissue factor pathway inhibitor-2 (TFPI-2) has previously been characterized as an endogenous anticoagulant. TFPI-2 is expressed in the vast majority of cells, mainly secreted into the extracellular matrix. Moreover, in sputum from cystic fibrosis patients TFPI-2 C-terminal fragments are generated and found associated with immunoglobulins. Together our data describe a previously unknown host defense mechanism and therapeutic importance of TFPI-2 against invading Gram-negative bacterial pathogens.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse TFPI-2 Protein (TFP-MM102) Specifications/Details

Protein
TFPI-2
Synonyms
TFPI-2; PP5; TFPI2; REF1; TFPI-2REF1
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Leu23-Ser230
Molecular Weight
The protein has a predicted MW of 24.87 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse TFPI-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse TFPI-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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