Name: Mouse TNFSF12/TWEAK Protein (TNF-MM612)
Brand: KactusBio
SKU: TNF-MM612-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-Rabbit Fc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ser46-His249
Other Available Proteins:

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Catalog #TNF-MM612

Size

100UG

500UG

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Mouse TNFSF12/TWEAK Protein (TNF-MM612) Summary

Product Description	Recombinant Mouse TNFSF12/TWEAK Protein is expressed from HEK293 with Rabbit Fc tag at the N-terminus.It contains Ser46-His249.
Product Background	Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Mouse TNFSF12/TWEAK Protein (TNF-MM612) Specifications/Details

Protein	TNFSF12/TWEAK
Synonyms	TNFSF12; APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
Accession	O54907
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser46-His249
Molecular Weight	The protein has a predicted MW of 48.95 kDa. Due to furin cleavage and glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200).
Product Tag	N-Rabbit Fc
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse TNFSF12, Rabbit Fc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.12 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).
	Mouse TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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