Mouse TNFSF12/TWEAK Protein (TNF-MM612)
Mouse TNFSF12/TWEAK Protein (TNF-MM612)
  • Express System: HEK293
  • Product Tag: N-Rabbit Fc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser46-His249
Catalog #TNF-MM612
Regular price $315.00
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Product Description
Recombinant Mouse TNFSF12/TWEAK Protein is expressed from HEK293 with Rabbit Fc tag at the N-terminus.It contains Ser46-His249.
Product Background
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
Product Category
Recombinant Protein
Target
TNFSF12/TWEAK
Synonyms
TNFSF12; APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ser46-His249
Molecular Weight
The protein has a predicted MW of 48.95 kDa. Due to furin cleavage and glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
N-Rabbit Fc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse TNFSF12, Rabbit Fc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.12 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
Mouse TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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