Phi29 DNA Polymerase (PHI-BE101) Summary
Product Description |
Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29, with strong strand displacement activity and high processivity (up to more than 70 kbp). The enzyme has inherent 3'-5' exonuclease activity and higher fidelity than most Taq enzymes. It is used for isothermal amplification in vitro. |
Phi29 DNA Polymerase (PHI-BE101) Specifications/Details
Synonyms |
Phi29 enzyme; Phi29 protein; Phi29 polymerase; ϕ29 dna polymerase; ϕ29 polymerase; ϕ29 enzyme |
Applications |
Multiple strand displacement amplification (MDA) Whole genome amplification (WGA) Rolling circle amplification |
Concentration |
10U/µL |
Unit Definition |
One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTPs into acid-insoluble substances within 10 minutes in a total reaction volume of 50µL at 30°C. |
Source |
E. coli strain carrying the phi29 DNA polymerase gene cloned from Bacillus subtilis phage phi29 |
Biotinylated |
no |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped on dry ice. |
Stability And Storage |
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
Validation Images
|
|
Rolling cycle amplification of pUC57 plasmid with phi29 DNA Polymerase, product was digested with BspQI and then analyzed with Agarose gel. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
