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SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY) Summary
Product Description |
Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685. |
Product Background |
A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
SARS-COV-2 Spike S1 (N501Y) Protein (COV-VM1SY) Specifications/Details
Protein |
Spike S1 |
Synonyms |
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Val16-Arg685 |
Molecular Weight |
The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. |
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SARS-COV-2 Spike S1 (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 90% as determined by SEC-HPLC. |
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